瘢痕成纤维细胞的钙网蛋白表达研究(2)
作者：佚名； 更新时间：2014-12-13
i等［10］曾利用体外结缔组织收缩模型发现增生性瘢痕组织来源的成纤维细胞在体外有着比正常皮肤成纤维细胞更强的迁移扩散能力，并认为其作用可能与细胞内肌动蛋白丝积聚有关。笔者在博士后研究工作阶段却发现在相同培养条件下增生性瘢痕组织来源的成纤维细胞除有着较强迁移扩散能力外，还具有高表达β1、α1、α2、α3及α4整合素粘附分子的特性［11］，从而提示瘢痕成纤维细胞的强迁移扩散能力与高表达整合素粘附分子有关；而从本实验对CRT在瘢痕成纤维细胞内的表达情况来看，细胞内其CRT表达并不属持续性表达，CRT的高表达期一般是在细胞的迁移扩散阶段，而当细胞融合后细胞内CRT表达甚少。但就与同一时期培养的正常皮肤成纤维细胞相比，瘢痕成纤维细胞内的CRT表达则显得更为丰富。因此，结合我们以往的研究工作结果，我们认为：瘢痕成纤维细胞异常的迁移扩散能力是细胞内丰富的CRT、整合素粘附分子表达及肌动蛋白丝积聚共同作用的结果，其中积聚的肌动蛋白丝是其异常迁移扩散的重要动力来源，而有效表达的CRT及整合素粘附分子则是细胞与细胞外间质之间迁移动力的传递媒介；另外，CRT的丰富表达对瘢痕成纤维细胞钙离子的储存、释放及其信号传导等也有着重要的意义。
参考文献
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4 Burns K, Opas M,Michalak M. Calreticulin inhibits glucocorticoid-but not cAMP-sensitive expression of tyrosine aminotransferase gene in cultrued McA-RH7777 hepatocytes. Mol Cell Biochem, 1997, 171:37-43.
5 Coppolino MG, Woodside MJ, Demaurex N, et al. Calreticulin is essential for integrin-mediated calcium signalling and cell adhesion. Nature, 1997, 386:843-847.
6 Eggleton P, Reid KB, Kishore U, et al. Clinical relevance of calreticulin in systemic lupus erythematosus. Lupus, 1997, 6:564-571.
7 Winisk A, Foster C. ICAM-1 expression in a spontaneously transformed human keratinocyte cell line:Characterization by a simple cell-ELISA assay. J Invest Dermal, 1992, 99:48-52.
8 Tredget EE, Nedelec B, Scott-PG, et al. Hypertrophic scars, keloids, and contractures. The cellular and molecular basis for therapy. Surg Clin North Am, 1997, 77:701-30.
9 Zhu Q, Zelinka P, White T, et al. Calreticulin-integrin bidirectional signaling complex. Biochem Biophys Res Commun, 1997, 232:354-358.
10 Tsai CY, Hata K, Torii S, et al. Contraction potency of hypertrophic scar-derived fibroblasts in a connective tissue model:in vitro analysis of wound contraction. Ann Plast Surg, 1995, 35:638-646.
11 赵烨德，何清濂，纪徐淮，等.瘢痕成纤维细胞整合素表达实验研究.第二军医大学学报，1998，19：330-332.
参考文献
1 Krause KH, Michalak M.Calreticulin. Cell, 1997, 88:439-443.
2 Tector M, Zhang Q, Salter RD. Beta 2-microglobulin and calnexin can independently promote folding and disulfide bond formation in class I histocompatiblity proteins. Mol Immunol, 1997, 34:401-408.
3 Liu H,Bowes RC, van-de-Water B, et al. Endoplasmic reticulum chaperones GRP78 and calreticulin prevent oxidative stress, Ca2+ disturbances, and cell death in renal epithelial cells. J Biol Chem, 1997, 272:21751-21759.
4 Burns K, Opas M,Michalak M. Calreticulin inhibits glucocorticoid-but not cAMP-sensitive expression of tyrosine aminotransferase gene in cultrued McA-RH7777 hepatocytes. Mol Cell Biochem, 1997, 171:37-43.
5 Coppolino MG, Woodside MJ, Demaurex N, et al. Calreticulin is essential for integrin-mediated calcium signalling and cell adhesion. Nature, 1997, 386:843-847.
6 Eggleton P, Reid KB, Kishore U, et al. Clinical relevance of calreticulin in systemic lupus erythematosus. Lupus, 1997, 6:564-571.
7 Winisk A, Foster C. ICAM-1 expression in a spontaneously transformed human keratinocyte cell line:Characterization by a simple cell-ELISA assay. J Invest Dermal, 1992, 99:48-52.
8 Tredget EE, Nedelec B, Scott-PG, et al. Hypertrophic scars, keloids, and contractures. The cellular and molecular basis for therapy. Surg Clin North Am, 1997, 77:701-30.
9 Zhu Q, Zelinka P, White T, et al. Calreticulin-integrin bidirectional signaling complex. Biochem Biophys Res Commun, 1997, 232:354-358.
10 Tsai CY, Hata K, Torii S, et al. Contraction potency of hypertrophic scar-derived fibroblasts in a connective tissue model:in vitro analysis of wound contraction. Ann Plast Surg, 1995, 35:638-646.
11 赵烨德，何清濂，纪徐淮，等.瘢痕成纤维细胞整合素表达实验研究.第二军医大学学报，1998，19：330-332.
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